Several of these links are to wikipedia which may not be the best source. Also, a more detailed review of chemical bonds and the molecular structure of proteins, lipids, and carbohydrates would ensure the student has the knowledge to understand the cellular processes.
While these are topics the student should know from general biology, they often have misconceptions that need to be corrected. This is especially true for protein structure and understanding the allosteric regulation that is discussed throughout the text. A list of important terms is provided at the end of each chapter however, they are not defined in a glossary.
For the most part, the text is accurate and well written. The sections on energy and enzyme kinetics are particularly well written with excellent examples. However, there are a few errors. For instance, hyperpolarization is the state when the membrane potential of a cell becomes more negative and an action potential is inhibited but it is defined in the text as an increase in depolarization that leads to an action potential.
There are a few other sections that need some clarification because they could lead to misconceptions. For example, antiporters and symporters are involved in the active transport of one molecule while an ion diffuses and this is only discussed in the context of facilitated diffusion.
Also, the sentence, "On the other hand, membrane proteins are responsible for the selective permeability of membranes In most sections, the figures are helpful, but without defined figure legends, a few are unclear. The text was recently updated so recent references are included. When needed, new references can be added easily so the longevity should be adequate. The text provides an excellent review of the history of molecular and cellular biology.
Although more details would improve the accuracy and clarity of the text as described previously, most sections are well written and free of undefined technical jargon.
Each chapter of the text follows the same format with an introduction, learning objectives, and key terms provided. The terminology is consistent and new terms are defined. The text is divided into chapters that are each divided into smaller sections with subheadings. Each section is of a reasonable length and the information is logically divided. The text begins with an overview of the cell theory and organelle function followed by a review of chemical bonds, macromolecules, and energy.
The next section discusses principles of molecular biology with chapters on DNA replication, transcription, gene regulation, and molecular techniques. The last section describes the cell membrane, cellular transport, cell signaling, cell cycle, and evolution. There are a few instances where examples were given that required knowledge that is discussed in later chapters.
For example, the tertiary structure of insulin is provided as an example of covalent bonds before protein folding is presented. The challenge questions are a great addition to the text but a few of them needed information from future chapters to completely answer the question.
Also, answers to these questions provided at the end of the text would increase learning. Overall, the organization and flow of information is good. There are no major problems with interface.
A few of the figures are a bit distorted but not to the point of distracting from the reading. There are a few typos and repeated sections of sentences but these are rare and do not really affect the overall reading. This text provides a basic summary of cellular and molecular processes that could be customized for an introductory cell or molecular course.
There are a few errors that do need corrected and then additional details could be added to supply the desired rigor. The voice over powerpoints are helpful and make the text more accessible. Overall, this text provides a workable foundation for an open access text that can be customized to meet the demands of any introductory cell molecular course. This text is a great introductory text that includes the basics of cell and molecular biology.
It would be a very useful introductory text. Each chapter includes appropriate video presentations which adds to comprehension. There is a theme There is a theme based on evolution utilized throughout each chapters with appropriate examples. Main points are reinforced with guided exercises for students. Although each chapter could stand alone it does seem that some reordering of chapters might help. There are some areas that are used but not explained until another chapter. But these are actually quite small.
Although the textbook us accurate it would be useful to include not only new terms but old venacular such as Krebs Cycle. Since depending on textbook students may run into both terms in later study. No textbook can be completely relevant forever but I did not find any current information not supported by links which help date the time content was written. It would be easy to provide links to any current new information and future information.
Whereas the text is very readable and flows nicely, there is no index or glossary provided. Main terms are listed at the end of each chapter but no glossary for accuracy. Plus no index makes it harder for students to find concepts they may need to review in later chapters.
I do like that learning objectives are clearly stated in each chapter. Whereas each chapter can be taken alone they flow well from one to another. The ease of readability and lends to a consistent understanding. Balance of new concepts is appropriate with not too much new information introduced at one time. It is quite easy to have each chapter as a stand alone section with the videos provided.
Topics that need information previously presented are represented in a shorter version. There is a logical progression that works well. The cellular biology is the last third of the book I would have liked to have seen some cellular presented earlier yet this format does work. I had absolutely no issues navigating throughout. Since I have not used a Open Textbook I was extremely happy because my skills are somewhat lacking.
If I can navigate I think most students will do so with ease. The illustrations needed more parts labeled and more views so students don't get stuck only being able to recognize one illustration view. This book covers the basics of cell and molecular biology and would be useful in an introductory class level. I expected more molecular techniques discussed since there was an entire chapter on DNA techniques however model organisms and I expected more molecular techniques discussed since there was an entire chapter on DNA techniques however model organisms and protein techniques were not covered in much detail if any.
The book supplies a list of key terms however there is no glossary or index. A major challenge in understanding the life sciences is grasping the jargon. Therefore, a glossary would be very helpful. The topics covered in this textbook are the topics expected to be covered in this subject area. Most of the information provided especially in the text were accurate. I found that some of the illustrations were lacking detail or had additional information which made them less accurate based on the text.
For example, when discussing the various subunits of a histone, the illustration for histones does not show these subunits. Also, when illustrating RNA polymerase, the tail is phosphorylated on the enzyme but there is no mention of that phosphorylation in the text how it is involved in the modification of mRNA. The technology portion of the textbook may need updating as new molecular techniques are developed.
In addition to the molecular techniques some of the studies used as examples could be updated. For example, there have been extensive studies in epigenetics done on mouse rearing and twin studies on schizophrenia which could be included in the text.
I found this to be too much information when the reader had not learned about translation yet. In addition to that statement I found some of the concepts mentioned felt out of order like the author discusses protein modifications after primary structures but before discussing secondary, tertiary and quaternary structures. There was also a lack of specific examples or real world application to these concepts. Protein motifs were discussed vaguely rather than giving a specific example where you might see coil-coil motifs.
Enzymes were discussed specifically from a biochemical perspectives and not a cellular perspective. There was no mention of how enzymes are found in various parts of the cells or how the cell is an extremely crowded place. In the DNA chapter the author mentions how size, shape and number of chromosomes is specific to species but does not give us an example or show us karyotypes from various species to clarify this statement.
This could continue the misconception that more complex animals have a higher number of chromosomes. Some of the illustrations were also confusing, for example the illustration for primase and heterochromatin and euchromatin are mentioned without an illustration. Finally, I love the idea of the challenges however I found some of the challenges confusing or inappropriate like the challenge on pg in which students are learning about chromosomes but getting asked specific about cell cycles.
This book is extremely consistent which I appreciate. Each chapter opens with a general overview and student learning objectives. Challenges are added intermittingly throughout the chapter and at the end of the chapter is a table of key terms. Each chapter all consists of voice over PowerPoints and external links for YouTube videos and websites to clarify the material.
There is modularity to each chapter and it is easy to break up readings. I did not like the way the topics were organized nor the flow of some topics. I would prefer for Cell and Molecular Biology to be presented as central dogma DNA-mRNA-proteins then get into gene regulation, cellular energetics, membrane structure and transport as well as additional chapters on cell signaling, before discussing cell cycle, cancer and motility.
I also found the last chapter felt out of place and portions of that chapter could be incorporated into each chapter of the book giving an overall evolution theme. I also found various topics felt out of place within the chapter. For example, telomerase was thrown into the DNA replication chapter before discussing the natural shortening of chromosomes after each replication cycle. Also, regulation of transcription was discussed before the details of transcription and regulating of protein turn over may be best placed either in the translation chapter or protein chapter rather than the gene regulation chapter.
I found the textbook easy to navigate although a hyperlinked index or a breakdown of a table of contents would be extremely helpful. Use a plotting software to produce a scatter plot of absorbance vs sugar concentration, and determine the equation of the straight line: this will be used to calculate the reducing sugar concentration in analytical samples using the absorbance measurements. Figure 4 shows standard curves for the same range of samples using different amounts of glucose in the DNSA working reagent.
With increasing amounts of glucose included, the linear range of the assay is expanded to cover lower sample concentrations, and the absorbance values increase, toward the limits of detection in a typical spectrophotometer. Absorbance values should be within 0. The of Enzyme Kinetics reactions should contain buffer, substrate, and enzyme, all at known concentrations. In the example given here, we utilized 50 mM sodium citrate buffer, an enzyme concentration of 4.
DNSA0: working reagent with no additional glucose. Kcat: Km: 0. Plot these values against substrate concentration, to generate a full kinetic plot for the reaction Fig. The DNSA method is particularly useful for analysis of endo type hydrolysis of polysaccharide substrates.
This is because the initial reducing sugar concentration in the substrate is low due to the length of the carbohydrate chains, and because endo chain cleavage generates a lot of new reducing ends. An exo type reaction, such as polysaccharide debranching, will generate comparatively fewer reducing ends, so additional glu- cose in the working reagent may be required. When performing an experiment, a great deal of preliminary scoping is required to establish a reagent composition which will give reliable, reproducible measurements over the range of reducing sugar concentrations under analysis.
It is impor- tant that the standard curve of absorbance vs reducing sugar concentration be performed using the same reagent condi- tions as the experiment. This initial scoping phase can be labo- rious, but is preferable to having to begin again with a different reagent composition mid-way through an experiment.
The most appropriate monosaccharide to use for your stan- dard curve will typically be the major component of your poly- saccharide substrate i. Different mono- saccharides give different responses to DNSA treatment. The addition of Rochelle salt is part of the inherited protocol in a lot of laboratories, but the decision to include this step varies, as the benefit is not always apparent. The addition of the Rochelle salt should help to stabilize the color change if a significant coloration has been achieved and if added at the correct moment, but can instead dilute a sample and cause loss of color at lower color intensities.
Ideally, the working reagent composition should be reported in full in any eventual publications arising from the work to allow for future replication and comparison of results. This is rarely seen in practice, but is a helpful detail to include in reports. References 1. Arch Biochem — Nature — 4. J Biol Chem — Open navigation menu. Close suggestions Search Search. User Settings. Skip carousel. Carousel Previous. Carousel Next. What is Scribd? Jump to Page.
Search inside document. McKee Abstract Use of the 3,5-dinitrosalicylic acid reagent allows the simple and rapid quantification of reducing sugars. The aldehyde or ketone functional group of a sugar is D. McKee Fig. McKee to stabilize the color change. McKee 4. McKee produces standard curves under a range of conditions, both with and without the use of the Rochelle salt stabilizer to become sufficiently familiar with its effects, before true experi- mental work begins.
Kommineni Gopi Krishna. Syahid Ashari. Kalana Jayatillake. Clint Charles P. Annie Chen. Mary Francia Rico. Delyana Ratnasari. Ruswanto Ruswanto. Elizabeth Warden. Popular in Organic Chemical. Anonymous iOYpj Noor Hasanah. Vincent Nguyen. Nikhil Art. Jessica Matos. Mayyank Garg. Biodiesel Pala Fredricksen. Ahmed Hamza. Diegowz Figuerz. Dee Lawan. Amna Batuul. Hendy Dwi Warmiko.
Entisa Mullaj. Quick navigation Home. Hughes 7 Test Bank. DuBrin 6 Instructor's Manual. DuBrin 6 Test Bank. DuBrin 7 Instructor's Manual. DuBrin 7 Test Bank. Feldman Instructor's Manual. Blanchard 6 Instructor's Manual. Norton 3 Solution Manual. Lussier 4. Rue, Lloyd L. Byars 13 Solution Manual.
Byars 13 Test Bank. Daft, Dorothy Marcic 7 Instructor's Manual. Daft, Dorothy Marcic 7 Test Bank. Maher 11 Solution Manual. Maher 11 Test Bank. Hilton 9. Hilton 9 Test Bank. Thomas ,S. Charles Maurice 9 Solution Manual. Froeb, Brian T. McCann 2 Solution Manual. McCann 2 Test Bank.
Bagley Instructor's Manual. Bagley Test Bank. Trevino 5 Instructor's Manual. Trevino 5. Snell, George W. Bohlander 16 Instructor's Manual. Bohlander 16 Test Bank. Brown, Daniel W. DeHayes 7 Solution Manual. DeHayes 7 Test Bank. Solomon 7 Solution Manual. Certo 12 Instructor's Manual. Hu Instructor's Manual. Timmons 8. Evans Instructor's Manual. Evans Test Bank.
Ivancevich 8 Solution Manual. Ivancevich 9 Solution Manual. Jones 6 Instructor's Manual. Knight Solution Manual 2. Anderson Solution Manual. Anderson Test Bank. Cunningham 6 Solution Manual. Das 7 Instructor's Solutions Manual. Das 7 Instrucotr's Manual. Mannering 4 Solution Manual. Stair, George Reynolds 10 Solution Manual. Stair, George Reynolds 10 Test Bank.
Gitman 12 Instructor's Manual. Gitman 13 Instructor's Manual. Ritter 12 Solution Manual. Ritter 12 Test Bank. Rejda 10 Solution Manual. Author : Robert F. Schleif File Size : Signal Transduction Immunohistochemistry Alexander E. Kalyuzhny — in Science. Author : Alexander E. Kalyuzhny File Size : Author : Alfons Navarro File Size : Coronaviruses Helena Jane Maier — in Science. Morphology Methods Ricardo V.
0コメント